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L4919

Sigma-Aldrich

Lisozima

BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein

Sinônimo(s):

Mucopeptídeo N-acetilmuramoil-hidrolase, Muramidase

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About This Item

Número CAS:
12650-88-3
Número da licença da enzima:
3.2.1.17 (BRENDA, IUBMB)
Número CE:
235-747-3
Número MDL:
MFCD00131557
Código UNSPSC:
12352204
NACRES:
NA.54

fonte biológica

chicken egg white

Nível de qualidade

200

grau

for molecular biology

linha de produto

BioUltra

Ensaio

≥98% (SDS-PAGE)

forma

lyophilized powder

atividade específica

≥40,000 units/mg protein

peso molecular

single-chain 14.3 kDa

composição

Protein, ≥90%

técnica(s)

cell based assay: suitable

adequação

suitable for cell lysis

aplicação(ões)

cell analysis

temperatura de armazenamento

−20°C

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Descrição geral

Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.

Aplicação

Lysozyme from chicken egg white has been used:
  • in dielectric spectroscopy studies of dynamics of protein
  • as a control to measure the human lysozyme activity
  • as a supplement in soaking solution to treat lenses

A enzima quebra as paredes celulares das bactérias; usada para preparar esferoplastos.

Ações bioquímicas/fisiológicas

A lisoenzima hidrolisa as ligações β(1→4) entre o ácido N-acetilmurâmico e os resíduos de N-acetil-D-glicosamina no peptidoglicano e entre resíduos de N-acetil-D-glicosamina na chitodextrina. Células gram-positivas são bastante susceptíveis a essa hidrólise, pois suas paredes celulares têm uma alta proporção de peptidoglicano. Bactérias gram-negativas são menos susceptíveis devido à presença de uma membrana externa e uma menor proporção de peptidoglicano. Contudo, essas células podem ser hidrolisadas na presença de EDTA que quela íons metálicos na membrana bacteriana externa.

A enzima fica ativa em um amplo intervalo de pH (6,0 a 9,0). Em pH 6,2, atividade máxima é observada ao longo de um intervalo de forças iônicas (0,02 a 0,100 M) que em pH 9,2 (0,01 a 0,06 M).
Lysozymes participate in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.

Características e benefícios

  • Highly purified by repeated crystallization and dialysis
  • Each lot is use-tested for isolation of plasmid DNA from E. coli

Definição da unidade

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

Nota de preparo

3× crystallized

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Sigma-Aldrich

L7386

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GenElute™ Bacterial Genomic DNA Kits sufficient for 70 purifications

Sigma-Aldrich

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GenElute™ Bacterial Genomic DNA Kits

Lysozyme human Lysobac™, recombinant, expressed in rice, ≥100,000 units/mg protein, lyophilized powder

Sigma-Aldrich

L1667

Lysozyme human

GenElute™ Bacterial Genomic DNA Kits sufficient for 10 purifications

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Lysozyme from human neutrophils ≥95% (SDS-PAGE), lyophilized powder, ≥100,000 units/mg protein (E1%/280)

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L8402

Lysozyme from human neutrophils

GenElute™ Bacterial Genomic DNA Kits sufficient for 350 purifications

Sigma-Aldrich

NA2120

GenElute™ Bacterial Genomic DNA Kits

SAFC

SAFC

1.05281

Lysozyme

Mutanolisina lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified

Sigma-Aldrich

M9901

Mutanolisina

Mutanolisina free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)

Sigma-Aldrich

SAE0092

Mutanolisina

Protein dynamics in a broad frequency range: Dielectric spectroscopy studies
Nakanishi M and Sokolov AP
Journal of Non-Crystalline Solids, 407, 478-485 (2015)
Natural Food Antimicrobial Systems (2000)
Metabolic engineering of probiotic Saccharomyces boulardii
Liu JJ, et al.
Applied and Environmental Microbiology, 82(8), 2280-2287 (2016)
Corneal cell adhesion to contact lens hydrogel materials enhanced via tear film protein deposition
Elkins CM, et al.
Testing, e105512-e105512 (2014)
Jordi van Gestel et al.
Nature ecology & evolution, 3(8), 1184-1196 (2019-07-25)
Microbes are exposed to changing environments, to which they can respond by adopting various lifestyles such as swimming, colony formation or dormancy. These lifestyles are often studied in isolation, thereby giving a fragmented view of the life cycle as a
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HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size

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Protocolos

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

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