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Documentos Principais

L2879

Sigma-Aldrich

Lysozyme chloride form from chicken egg white

Grade VI, ≥35,000 units/mg protein (E1%/282)

Sinônimo(s):

Peptidoglycan iV-acetylmuramoyl hydrolase, Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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About This Item

Número CAS:
Número da licença da enzima:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

fonte biológica

chicken egg white

Nível de qualidade

tipo

Grade VI

Formulário

lyophilized powder

atividade específica

≥35,000 units/mg protein (E1%/282)

peso molecular

~14.3 kDa

composição

Protein, ≥80%

técnica(s)

cell based assay: suitable

adequação

suitable for cell lysis

aplicação(ões)

cell analysis

temperatura de armazenamento

−20°C

Descrição geral

Lysozyme, also known as muramidase, is a bacteriolytic enzyme found in phagocytic cells like leukocytes. The name "lysozyme" is derived from its ability to dissolve or hydrolyze the β-1-4-glycosidic bond between N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) in the walls of certain bacteria. This protein is present in animals, plants, bacteria, and viruses. It can also be found in granules of neutrophils, macrophages, as well as in serum, saliva, milk, honey, and hen egg white. There are different types of lysozymes, including the c-type (chicken type), the g-type (goose-type), and the i-type (invertebrates).

Aplicação

Lysozyme chloride form from chicken egg white has been used:
  • as a component in the protein mixture to study the effects of yeast cells on membrane fouling caused by a protein mixture in dead-end filtration
  • in dip system to study its bacteriostatic or bactericidal effects on Salmonella-contaminated broiler parts
  • in viscometric assay for determining lysozyme activity    

Ações bioquímicas/fisiológicas

Lysozyme serves as an indicator of macrophage-mediated host response, correlates with white cell death, and exhibits a high turnover rate. Elevated levels of serum lysozyme have been observed in various chronic inflammatory conditions, inflammatory bowel diseases, hematological disorders, and renal disorders. The c-type lysozyme from hen egg white is commonly used as a model for studying protein structure and function. Muramidase primarily exhibits bactericidal activity against Gram-positive bacteria.

Definição da unidade

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

forma física

Lyophilized powder containing sodium chloride and sodium acetate

Nota de preparo

3× Crystallized

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Artigos

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Protocolos

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

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