L2879
Lysozyme chloride form from chicken egg white
Grade VI, ≥35,000 units/mg protein (E1%/282)
Sinônimo(s):
Peptidoglycan iV-acetylmuramoyl hydrolase, Mucopeptide N-acetylmuramoylhydrolase, Muramidase
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About This Item
Produtos recomendados
fonte biológica
chicken egg white
Nível de qualidade
tipo
Grade VI
Formulário
lyophilized powder
atividade específica
≥35,000 units/mg protein (E1%/282)
peso molecular
~14.3 kDa
composição
Protein, ≥80%
técnica(s)
cell based assay: suitable
adequação
suitable for cell lysis
aplicação(ões)
cell analysis
temperatura de armazenamento
−20°C
Descrição geral
Lysozyme, also known as muramidase, is a bacteriolytic enzyme found in phagocytic cells like leukocytes. The name "lysozyme" is derived from its ability to dissolve or hydrolyze the β-1-4-glycosidic bond between N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) in the walls of certain bacteria. This protein is present in animals, plants, bacteria, and viruses. It can also be found in granules of neutrophils, macrophages, as well as in serum, saliva, milk, honey, and hen egg white. There are different types of lysozymes, including the c-type (chicken type), the g-type (goose-type), and the i-type (invertebrates).
Aplicação
Lysozyme chloride form from chicken egg white has been used:
- as a component in the protein mixture to study the effects of yeast cells on membrane fouling caused by a protein mixture in dead-end filtration
- in dip system to study its bacteriostatic or bactericidal effects on Salmonella-contaminated broiler parts
- in viscometric assay for determining lysozyme activity
Ações bioquímicas/fisiológicas
Lysozyme serves as an indicator of macrophage-mediated host response, correlates with white cell death, and exhibits a high turnover rate. Elevated levels of serum lysozyme have been observed in various chronic inflammatory conditions, inflammatory bowel diseases, hematological disorders, and renal disorders. The c-type lysozyme from hen egg white is commonly used as a model for studying protein structure and function. Muramidase primarily exhibits bactericidal activity against Gram-positive bacteria.
Definição da unidade
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
forma física
Lyophilized powder containing sodium chloride and sodium acetate
Nota de preparo
3× Crystallized
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Artigos
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Protocolos
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
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