L2650

Lectin from Glycine max (soybean)

peroxidase conjugate, lyophilized powder

• Sinônimo(s): SBA, Soybean agglutinin
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: Glycine max (soybean)
• conjugado: peroxidase conjugate
• Ensaio: 85.00-100.00% (Warburg-Christian)
• Formulário: lyophilized powder
• potência: <80 μg per mL agglutination activity
• peroxidase activity: 25-100 units/mg protein
• composição: Protein, ~95% modified Warburg-Christian
• técnica(s): western blot: suitable
• nº de adesão UniProt: P05046
• temperatura de armazenamento: −20°C
• Informações sobre genes: soybean ... LE1(100818710)

Descrição

Aplicação

General Western Blot Protocol:

• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

1. Load samples at 500 ng of glycoprotein per lane
2. Run 4-20% Bis-Tris SDS page gel
3. Transfer gel to a PVDF membrane
4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
7. Detect using chemiluminescent substrate (CPS1-120)

Ações bioquímicas/fisiológicas

SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.

Definição da unidade

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0, at 20 °C.

forma física

Contains sodium citrate

Nota de preparo

Conjugates are prepared from affinity purified lectin.

Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Nota de análise

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

Informações de segurança

• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)