KCQS01
KiCqStart® SYBR® Green qPCR ReadyMix™
Low ROX™, for ABI and Stratagene instruments
Sinônimo(s):
qPCR master mix, sybr green qPCR
About This Item
Produtos recomendados
forma
liquid
uso
sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions
Características
dNTPs included
hotstart
condição de armazenamento
protect from light
técnica(s)
qPCR: suitable
cor
colorless
entrada
purified DNA
compatibilidade
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI ViiA 7
for use with Agilent AriaMx
for use with Douglas Scientific IntelliQube
for use with Qiagen Rotor-Gene Q
for use with QuantStudio™
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000
método de detecção
SYBR® Green
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
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Descrição geral
Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.
Aplicação
- for the amplification and quantification of DNA in real-time PCR (qPCR) assay
- in the amplification and quantification of transcripts in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR)
- in the amplification of complementary DNA (cDNA) by real-time PCR (qPCR) assay
- Gene expression
- DNA quantification
- CHiP
Características e benefícios
- Assay results in as little as 33 minutes
- Highly efficient and sensitive real-time PCR results
- Little/no optimization required
Componentes
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
Qualidade
Outras notas
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
Informações legais
produto relacionado
recomendado
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Artigos
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
Ago RIP to Isolate microRNA and their Targets Using Imprint® RNA Immunoprecipitation Kit
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
Protocolos
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
Once an assay has been optimized, it is important to verify the reaction efficiency. This information is important when reporting and comparing assays. In this example protocol, the assay efficiency is compared over a wide and narrow dynamic range of cDNA concentrations.
Gradient PCR for assay optimization is to determine the optimum annealing temperature (Ta) of the primers by testing identical reactions containing a fixed primer concentration, across a range of annealing temperatures.
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.
Conteúdo relacionado
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
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