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KCQS01

Sigma-Aldrich

KiCqStart® SYBR® Green qPCR ReadyMix™

Low ROX™, for ABI and Stratagene instruments

Sinônimo(s):

qPCR master mix, sybr green qPCR

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About This Item

Código UNSPSC:
41106300
NACRES:
NA.55

forma

liquid

uso

sufficient for 1250 reactions
 sufficient for 250 reactions
 sufficient for 5000 reactions

Características

dNTPs included
hotstart

condição de armazenamento

protect from light

técnica(s)

qPCR: suitable

cor

colorless

entrada

purified DNA

compatibilidade

for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI ViiA 7
for use with Agilent AriaMx
for use with Douglas Scientific IntelliQube
for use with Qiagen Rotor-Gene Q
for use with QuantStudio™
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

método de detecção

SYBR® Green

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

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Descrição geral

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.

Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.

Aplicação

KiCqStart® SYBR® Green qPCR ReadyMix™ has been used:
  • for the amplification and quantification of DNA in real-time PCR (qPCR) assay
  • in the amplification and quantification of transcripts in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR)
  • in the amplification of complementary DNA (cDNA) by real-time PCR (qPCR) assay
PCR applications:
  • Gene expression
  • DNA quantification
  • CHiP

Características e benefícios

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required

Componentes

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, ROX Reference Dye (for 580-585 nm excitation), and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Qualidade

Kit components are free of contaminating DNase and RNase. KiCqStart® SYBR® Green qPCR ReadyMix™, Low ROX is functionally tested in qPCR. Kinetic analysis must demonstrate linear resolution over six orders of dynamic range (r2 > 0.995) and a PCR efficiency > 90%.

Outras notas

Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

Informações legais

6538 is a trademark of American Type Culture Collection
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
Mx3000P is a registered trademark of Agilent Technologies, Inc.
Mx3005P is a registered trademark of Agilent Technologies, Inc.
Mx4000 is a trademark of Agilent Technologies, Inc.
QuantStudio is a trademark of IROA Technologies LLC
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Rotor-Gene is a registered trademark of Qiagen GmbH
SYBR is a registered trademark of Life Technologies

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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