H8651

HEPES

BioXtra, ≥99.0% (titration)

• Sinônimo(s): Ácido 4-(2-hidroxietil)piperazina-1-etanossulfônico, Ácido N-(2-hidroxietil)piperazina-N′-2-etanossulfônico
• Fórmula empírica (Notação de Hill): C₈H₁₇N₂NaO₄S
• Número CAS: 75277-39-3
• Peso molecular: 260.29
• Número CE: 278-169-7
• Número MDL: MFCD00036463
• Código UNSPSC: 12161700
• ID de substância PubChem: 24895527
• NACRES: NA.25

Propriedades

• linha de produto: BioXtra
• Ensaio: ≥99.0% (titration)
• forma: powder
• Impurezas: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• pH: 10.0-12.0 (1 M in H₂O)
• faixa de pH útil: 6.8-8.2
• pKa (25 °C): 7.5
• solubilidade: H₂O: 1 M, clear, colorless
• traços de ânion: chloride (Cl^-): ≤0.05%; sulfate (SO₄^2-): ≤0.05%
• traços de cátion: Al: ≤0.0005%; Ca: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Pb: ≤0.001%; Zn: ≤0.0005%
• absorção: ≤0.2 at 260 in H₂O at 1 M; ≤0.2 at 280 in H₂O at 1 M
• aplicação(ões): diagnostic assay manufacturing
• cadeia de caracteres SMILES: [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• chave InChI: RDZTWEVXRGYCFV-UHFFFAOYSA-M

Descrição

Descrição geral

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Aplicação

HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Outras notas

Easily compare specifications for HEPES sodium salt products with the HEPES sodium salt specification table.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)