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RDD035

Sigma-Aldrich

HEPES

≥99.5% (titration), free-flowing, Redi-Dri™

Sinônimo(s):

Ácido 4-(2-hidroxietil)piperazina-1-etanossulfônico, Ácido N-(2-hidroxietil)piperazina-N′-2-etanossulfônico

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About This Item

Fórmula empírica (Notação de Hill):
C8H17N2NaO4S
Número CAS:
75277-39-3
Peso molecular:
260.29
Número MDL:
MFCD00036463
Código UNSPSC:
12161700
NACRES:
NA.25

linha de produto

Redi-Dri™

Nível de qualidade

100

Ensaio

≥99.5% (titration)

qualidade

free-flowing

faixa de pH útil

6.8-8.2

pKa (25 °C)

7.5

aplicação(ões)

diagnostic assay manufacturing

cadeia de caracteres SMILES

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

chave InChI

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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Descrição geral

HEPES sodium salt is essential to prepare HEPES buffer that is commonly used for cell culture and protein applications. It is useful for maintaining homeostasis when working with cells outside of the incubator by balancing CO2. HEPES buffering capacity exceeds most buffers, with a pKa of 7.5, making it optimal for maintaining pH in biological systems. In solution, it is non-toxic, zwitterion with a physiological buffering range of pH 6.8 - 8.2. It has a low metal binding capacity and is safe to use in research for metal-binding proteins.

Aplicação

HEPES sodium salt can be used:
  • as a protein precipitation buffer
  • in cell culture media
  • as a binding buffer

Características e benefícios

  • Redi-Dri™ eliminates clumping for hygroscopic chemicals
  • powder remains free-flowing and easy to use
  • Safer to handle and weigh out for standard use in lab
  • Eliminates clumps, maintains product moisture, quality, and characteristics

forma física

Lyophilized, spray dried, or crystallized material.

Outras notas

For additional information on our range of Biochemicals, please complete this form.

Informações legais

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
SLCF3906

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Nishant Mehta et al.
Cell reports, 28(10), 2509-2516 (2019-09-05)
V-domain immunoglobulin (Ig) suppressor of T cell activation (VISTA) is an immune checkpoint protein that inhibits the T cell response against cancer. Similar to PD-1 and CTLA-4, a blockade of VISTA promotes tumor clearance by the immune system. Here, we report a
Åsa Birna Birgisdottir et al.
Autophagy, 15(8), 1333-1355 (2019-02-16)
Autophagosome formation depends on a carefully orchestrated interplay between membrane-associated protein complexes. Initiation of macroautophagy/autophagy is mediated by the ULK1 (unc-51 like autophagy activating kinase 1) protein kinase complex and the autophagy-specific class III phosphatidylinositol 3-kinase complex I (PtdIns3K-C1). The
Peng Yang
Macromolecular bioscience, 12(8), 1053-1059 (2012-06-19)
When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"
Hubert Gojzewski et al.
Scanning, 34(3), 159-169 (2011-09-29)
In this study, we deposited isolated magnetosomes from magnetotactic bacteria Magnetospirillum strain AMB-1 onto solid surfaces using spin coating (SC) and drop coating (DC) techniques. Four imaging techniques have been used to visualize the sample structure: scanning and transmission electron
Anca G Delgado et al.
Microbial cell factories, 11, 128-128 (2012-09-15)
Buffering to achieve pH control is crucial for successful trichloroethene (TCE) anaerobic bioremediation. Bicarbonate (HCO3-) is the natural buffer in groundwater and the buffer of choice in the laboratory and at contaminated sites undergoing biological treatment with organohalide respiring microorganisms.
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Redi-Dri™ – The World of Free-flowing Hygroscopic Products

Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

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