H3537

HEPES

99.5%, liquid, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

• Sinônimo(s): Ácido N-(2-hidroxietil)piperazina-N′-2-etanossulfônico
• Número CAS: 7365-45-9
• Número MDL: MFCD00006158
• Código UNSPSC: 12161700
• ID de substância PubChem: 329815199
• NACRES: NA.25

Propriedades

• Nome do produto: HEPES, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered
• grau: BioPerformance Certified
• Nível de qualidade: 200
• esterilidade: 0.2 μm filtered
• Formulário: liquid
• concentração: 1 M
• técnica(s): cell culture | mammalian: suitable
• Impurezas: Bioburden, tested; DNase, RNase, Protease, Nickase, free; endotoxin, tested
• pH: 5.0-6.0
• faixa de pH útil: 6.8-8.2
• traços de cátion: Fe: <5 ppm; heavy metals (as Pb): <5 ppm
• adequação: suitable for molecular biology
• aplicação(ões): diagnostic assay manufacturing
• cadeia de caracteres SMILES: OCCN1CCN(CCS(=O)(O)=O)CC1
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• chave InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Descrição

Descrição geral

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicação

RNAse HEPES has been used:

• To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
• To recover purified ribonucleotide
• As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
• As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable