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H2663

Sigma-Aldrich

Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal

clone HDAC2-62, purified from hybridoma cell culture

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About This Item

Número MDL:
MFCD02096544
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

200

conjugado

unconjugated

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

HDAC2-62, monoclonal

forma

buffered aqueous solution

peso molecular

antigen ~55 kDa

reatividade de espécies

human, chicken, bovine, canine, rat, mouse

embalagem

antibody small pack of 25 μL

técnica(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using total cell extracts from NIH3T3 cells

Isotipo

IgG2b

nº de adesão UniProt

Q92769

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... HDAC2(3066)
mouse ... Hdac2(15182)
rat ... Hdac2(84577)

Descrição geral

Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the HDAC2-62 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C-terminal region of human histone deacetylase 2 (amino acid residues with N-terminal added cysteine) conjugated to maleimide-activated KLH. Histone deacetylase 2 (HDAC2) is the catalytic subunits of different multiprotein regulatory complexes.

Especificidade

Monoclonal Anti-Histone Deacetylase 2 (HDAC2) recognizes human, bovine, dog, rat, mouse, and chicken HDAC2 (approx. 55 kDa). The antibody epitope resides within amino acids 471-488 of human HDAC2.

Aplicação

Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal has been used in:
  • co-immunoprecipitation
  • chromatin immunoprecipitation (ChIP)
  • western blot
  • immunohistochemistry
  • enzyme linked immunosorbent assays (ELISA)

Ações bioquímicas/fisiológicas

Histone Deacetylases including HDAC′s 1-10 facilitate transcriptional repression and nucleosome condensation. Abnormal recruitment of HDACs by various oncoproteins may occur in certain neoplastic diseases. Inhibition of HDAC2 activity by valproic acid induces proteasomal degradation of HDAC2.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Dimethyl fumarate regulates histone deacetylase expression in astrocytes
Kalinin S, et al.
Journal of Neuroimmunology, 263 (2013)
Jeyran Shahbazi et al.
Oncotarget, 5(12), 4257-4268 (2014-06-24)
Myc oncoproteins and histone deacetylases (HDACs) exert oncogenic effects by modulating gene transcription. Paradoxically, N-Myc induces p53 gene expression. Tumor protein 53-induced nuclear protein 1 (TP53INP1) phosphorylates p53 protein at serine 46, leading to enhanced p53 activity, transcriptional activation of
Histone Deacetylases: A Saga of Perturbed Acetylation Homeostasis in Cancer
Parbin S, et al.
The Journal of Histochemistry and Cytochemistry, 62 (2014)
Insights into the activation mechanism of class I HDAC complexes by inositol phosphates
Watson PJ, et al.
Nature Communications, 7 (2016)
Stem Cell Factor SALL4 Represses the Transcriptions of PTEN and SALL1 through an Epigenetic Repressor Complex
Lu J, et al.
Testing, 4 (2009)
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