GE28-4082-53

HiTrap^® MabSelect^™

Cytiva 28-4082-53, pack of 5 × 1 mL

• Código UNSPSC: 23151816
• NACRES: NA.56

Propriedades

• ligand: recombinant protein A (E. coli)
• embalagem: pack of 5 × 1 mL
• fabricante/nome comercial: Cytiva 28-4082-53
• condição de armazenamento: (20% Ehtanol); (20% Ethanol)
• Parâmetros: 42 psi
• tamanho do leito: 7 mm × 25 mm
• volume do leito: 1 mL
• D.I. da coluna: 7 mm
• matriz: highly cross-linked agarose
• Tamanho médio da partícula: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-12
• working range: 3-10
• capacidade: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• adequação: suitable for bioprocess medium
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiTrap^® MabSelect^™ columns are prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Aplicação

Lab Purification using the MabSelect^™ Protein A tag for capture of antibodies.

Características e benefícios

• HiTrap^® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Outras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informações legais

HiTrap is a registered trademark of Cytiva

MabSelect is a trademark of Cytiva

Informações de segurança

• Pictogramas: GHS02
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de classe de armazenamento: 3 - Flammable liquids