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Key Documents

GE17-5132-03

Glutathione Sepharose 4 Fast Flow

Cytiva 17-5132-03, pack of 500 mL

Sinônimo(s):

Glutathione Column, Sepharose 4 Fast Flow

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

glutathione and 10-carbon linker arm

embalagem

pack of 500 mL

fabricante/nome comercial

Cytiva 17-5132-03

matriz

highly cross-linked 4% agarose

diâmetro médio

90 μm

cleaning

3-12

working range

3-12

capacidade

>10 mg/mL binding capacity

Descrição geral

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose 4 Fast Flow, 500 mL
Glutathione Sepharose 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.

Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose 4 Fast Flow provides good flow properties and is excellent for scaling up.

Características e benefícios

  • Fast, one-step purification of GST-tagged proteins
  • Mild elution conditions preserving protein antigenicity and function
  • For scale-up due to high flow properties
  • Glutathione coupled to Sepharose 4 Fast Flow via a 10-carbon spacer arm

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


Certificados de análise (COA)

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Artigos

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from GE Healthcare.

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Protocolos

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.

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