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Documentos Principais

GE17-0963-03

Protein A Sepharose™ Cl-4B

Cytiva 17-0963-03, pack of 25 mL

Sinônimo(s):

Protein A Column, Sepharose 4B

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

native protein A

embalagem

pack of 25 mL

fabricante/nome comercial

Cytiva 17-0963-03

condição de armazenamento

(20% Ehtanol)

Matriz

4% cross-linked agarose

tamanho de partícula

45-165 μm

diâmetro médio

90 μm

cleaning

2-10

Faixa de trabalho

3-9

capacidade

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

temperatura de armazenamento

2-8°C

Descrição geral

Protein A Sepharose™ CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.

Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Características e benefícios

  • Well documented for immunoglobulin purification and fractionation
  • Strong binding to Fc region of IgG
  • High capacity - 20 mg human IgG/mL.
  • Suited for immunoprecipitation procedures

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Sepharose is a trademark of Cytiva

Pictogramas

Flame
GHS02

Palavra indicadora

Warning

Frases de perigo

H226

Código de classe de armazenamento

3 - Flammable liquids

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Certificados de análise (COA)

Lot/Batch Number

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