GE17-0963-02
Protein A Sepharose™ Cl-4B
Cytiva 17-0963-02, pack of 500 mL
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About This Item
Código UNSPSC:
41106500
NACRES:
NA.56
Produtos recomendados
ligand
native protein A
embalagem
pack of 500 mL
fabricante/nome comercial
Cytiva 17-0963-02
condição de armazenamento
(20% Ehtanol)
matriz
4% cross-linked agarose
tamanho de partícula
45-165 μm
diâmetro médio
90 μm
cleaning
2-10
working range
3-9
capacidade
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
temperatura de armazenamento
2-8°C
Categorias relacionadas
Descrição geral
Protein A Sepharose™ CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Características e benefícios
- Well documented for immunoglobulin purification and fractionation
- Strong binding to Fc region of IgG
- High capacity - 20 mg human IgG/mL.
- Suited for immunoprecipitation procedures
Armazenamento e estabilidade
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Nota de análise
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Informações legais
Sepharose is a trademark of Cytiva
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Código de classe de armazenamento
3 - Flammable liquids
Certificados de análise (COA)
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