G5768
Gamborg′s B-5 Basal Salt Mixture
fine powder, suitable for plant cell culture
Sinônimo(s):
B-5 Salt Mixture, Basal Salt Mixture, Gamborg B-5 Medium
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.72
Produtos recomendados
Formulário
fine powder
Nível de qualidade
técnica(s)
cell culture | plant: suitable
aplicação(ões)
agriculture
temperatura de armazenamento
2-8°C
Descrição geral
Gamborg′ B-5 medium described by Gamborg, et al. may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis.
Aplicação
Gamborg′s B-5 Basal Salt Mixture has been used:
- as a constituent during the preparation of TEX buffer
- as a media to maintain potato suspension and callus cultures
- as a media in the primary-node transformation method
Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
Variante da fórmula
With the concentrations of macro- and micronutrients as described by Gamborg, et al. (1968).
Media Formulation
Media Formulation
Quantidade
Formulated to contain 3.1 grams of powder per liter of medium.
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Ox. Sol. 3
Código de classe de armazenamento
5.1B - Oxidizing hazardous materials
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Cell suspension cultures of Linum album were developed from internode portions of in vitro germinated plant in Gamborg's B5 medium supplemented with 0.4 mg naphthalene acetic acid/l. The highest biomass was 8.5 g/l with podophyllotoxin and 6-methoxypodophyllotoxin at 29 and
Artigos
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
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