G3776

Guanosine 5′-triphosphate sodium salt solution

HPLC purified, aqueous solution for RNA polymerase transcription

• Sinônimo(s): GTP
• Número CAS: 36051-31-7
• Número MDL: MFCD00077781
• Código UNSPSC: 41106305
• ID de substância PubChem: 24895138
• NACRES: NA.52

Propriedades

• fonte biológica: Porcine brain; bacterial (Corynebacterium); yeast
• Nível de qualidade: 300
• Ensaio: ≥95% (HPLC)
• Formulário: liquid
• concentração: 100 mM in H₂O (adjusted to pH 7)
• cor: colorless
• atividade externa: DNase, RNase and Nickase, none detected
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1
• InChI: 1S/C10H16N5O14P3.3Na/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;/q;3*+1/p-3/t3-,5-,6-,9-;;;/m1.../s1
• chave InChI: KZRMTEVIDYXWQW-CYCLDIHTSA-K
• Informações sobre genes: human ... HRAS(3265)

Descrição

Aplicação

Guanosine 5′-triphosphate sodium salt solution has been used:

• for reconstituting transducin during purification of transducin.
• as a compnent of the 3.3X loading buffer to assemble 32P-miR-31—3xFLAG-AGO2 complexes
• as a component of the nucleotide substrate mix to evaluate the hydrolytic activity of MutT homolog 1 (MTH1) on methylated nucleoside triphosphates
• in DNA-dependent RNA polymerase transcription

Ações bioquímicas/fisiológicas

Guanosine 5′-triphosphate (GTP) forms nearly 25% of the total nucleotide triphosphate pool and is a nucleotide anion. It participates in several biological functions such as cell signaling by activation of GTP-binding proteins, RNA and protein synthesis, and production of cyclic guanosine monophosphate (cGMP), a secondary messenger. GTP also participates in the production of stringent alarmone.

Informações de segurança

• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Faceshields, Gloves, type N95 (US)