D0180
Monoclonal Anti-DOPA Decarboxylase (DDC) antibody produced in mouse
clone DDC-109, ascites fluid
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fonte biológica
mouse
Nível de qualidade
conjugado
unconjugated
forma do anticorpo
ascites fluid
tipo de produto de anticorpo
primary antibodies
clone
DDC-109, monoclonal
peso molecular
antigen ~50 kDa
contém
15 mM sodium azide
reatividade de espécies
rabbit, sheep, guinea pig, monkey, rat, human, bovine, canine
técnica(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using bovine brain extract
Isotipo
IgG2b
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... DDC(1644)
rat ... Ddc(24311)
Descrição geral
DDC is a 85-90 kDa molecular weight protein that is mainly expressed in brain, kidney and liver.
Monoclonal Anti-DOPA Decarboxylase (DDC) (mouse IgG2b isotype) is derived from the DDC-109 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Aplicação
Monoclonal Anti-DOPA Decarboxylase (DDC) antibody produced in mouse may also be used in immunoprecipitation, immunocytochemistry and ELISA applications. In immunocytochemical labeling of brain tissue, the product stains the monoaminergic cells and proximal dendrites, but not cortical fibers. It is suitable for use in western blotting at a working dilution of 1:1000 using bovine brain extract. It was used for immunoblotting and immunoprecipitation of AADC (aromatic amino acid decarboxylase) from rat striatum in a study. It was used as a primary antibody for western blotting and co-immunoprecipitation studies using human embryonic kidney cells and human neuroblastoma cells.
Ações bioquímicas/fisiológicas
DOPA Decarboxylase (DDC), which requires pyridoxal phosphate for its activity, decarboxylates 5-hydroxytryptophan to form serotonin. It also decarboxylates L-DOPA to form dopamine in both dopaminergic and noradrenergic neurons.
DOPA decarboxylase (DDC, EC 4.1.1.28), catalyzes the decarboxylation of the 5-hydroxytrptophan (5-HTP) to 5-HT. It requires pyridoxal phosphate for its activity. This is the enzyme that also decarboxylates L-DOPA to form dopamine in both dopaminergic and noradrenergic.. Antibodies that react specifically with the enzyme enable the identification of both noradrenergic, dopaminergic, and serotoninergic systems, since DDC is present and active in all three types of neurons.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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