CWA-1143

Anti-Human CD47 (MEM-122) ColorWheel^® Dye-Ready mAb

• Sinônimo(s): Leukocyte surface antigen CD47;Integrin-associated protein
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: MEM-122, monoclonal
• linha de produto: ColorWheel^®
• Formulário: lyophilized
• peso molecular: calculated mol wt 35.19 kDa
• purificado por: affinity chromatography
• reatividade de espécies: human
• reatividade da espécie (prevista por homologia): nonhuman primates
• embalagem: antibody small pack of 25 μL
• características do produto alternativo mais ecológico: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• técnica(s): flow cytometry: suitable
• Isotipo: IgM
• sequência de epítopo: Extracellular domain
• nº de adesão de ID de proteína: Q08722
• nº de adesão UniProt: A0A0D9R1W9
• compatibilidade: for use with ColorWheel^® Dyes (Required, (Sold Separately))
• categoria alternativa mais ecológica: , Aligned
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: Monkey ... CD47(103228373)

Descrição

Descrição geral

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Especificidade

Clone MEM-122 is a mouse monoclonal antibody that detects CD47. It targets an epitope within the extracellular domain.

Imunogênio

COS-7 cells

Aplicação

Quality Control Testing

Evaluated by Flow Cytometry in A431 cells.

Flow Cytometry Analysis: Staining of one million A431 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million human peripheral blood lymphocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Flow Cytometry Analysis: Staining of one million A431 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Flow Cytometry Analysis: Staining of one million human peripheral blood lymphocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibilidade

Requires ColorWheel^® Dye (Sold Separately)

Descrição-alvo

Leukocyte surface antigen CD47 (UniProt: A0A0D9R1W9; also known as Integrin-associated protein, CD47) is encoded by the CD47 gene (Gene ID: 103228373) in Monkey. CD47 is a multi-pass transmembrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature form. It is widely expressed on all hematopoietic cells and most other cell types. The ligands of CD47 include SIRP , thrombospondin-1 (TSP-1), and integrins including vβ3 and 2β1. It plays a role in vβ3-mediated cell adhesion and Ca2+ influx, as well as in platelet activation via interaction with thrombospondin-1. In addition, CD47 modulates peripheral mononuclear (PMN) neutrophil migration across endothelial and epithelial monolayers. The rate of fMLP-stimulated PMN migration is shown to be decreased by CD47 functional blockage upon neutralizing antibody treatment, which can be partially reversed by PI3K inhibition. Following fMLP stimulation and after PMN transmigration, secondary granules-derived CD47 molecules are shown to accumulate on plasma membrane, resulting in increased cell surface CD47 immunoreactivity. Overexpression of CD47 is reported on the surface of many types of cancer cells where it forms a signaling complex with signal-regulatory protein (SIRP ), enabling the escape of these cancer cells from macrophage-mediated phagocytosis. (Ref.: Zhang, W., et al. (2020). Front. Immunol. 11; 18).

forma física

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstituição

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Informações legais

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable