Pular para o conteúdo
Merck
Todas as fotos(1)

Key Documents

View All Documentation

CS0760

Sigma-Aldrich

Isolated Mitochondria Staining Kit

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Número CE:
217-896-6
Código UNSPSC:
12352207
NACRES:
NA.32

Nível de qualidade

200

uso

 kit sufficient for 1000 reactions (using 96 multiwell plates)
 kit sufficient for 50 reactions (in a 2 mL cuvette)

embalagem

pkg of 1 kit

condição de armazenamento

dry at room temperature

técnica(s)

flow cytometry: suitable
protein staining: suitable

fluorescência

λex 490 nm; λem 590 nm (JC-1 dye)

aplicação(ões)

cell analysis
detection

método de detecção

fluorometric

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Categorias relacionadas

Aplicação

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.

Características e benefícios

  • Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
  • Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
  • Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

Princípio

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.

Nota de análise

The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

Somente componentes do kit

Nº do produto
Descrição
  • DMSO 1 mL
  • JC-1 Assay Buffer 5X 25 mL
  • JC-1 Stain 25 μg
  • Valinomycin Ready Made 100 μL

Pictogramas

Skull and crossbones
GHS06

Palavra indicadora

Danger

Frases de perigo

H300 + H310

Classificações de perigo

Acute Tox. 1 Dermal - Acute Tox. 1 Oral

Código de classe de armazenamento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Os clientes também visualizaram

Slide 1 of 3

1 of 3

Mito Red suitable for fluorescence, ≥70% (HPLC)

Sigma-Aldrich

53271

Mito Red

Cytochrome c Reductase (NADPH) Assay Kit 1 kit sufficient for 100 tests, determining cytochrome c reductase activity

Sigma-Aldrich

CY0100

Cytochrome c Reductase (NADPH) Assay Kit

Endoplasmic Reticulum Isolation Kit isolation of intact ER from mammalian soft tissues and cultured cells

Sigma-Aldrich

ER0100

Endoplasmic Reticulum Isolation Kit

Differential expression of choline kinase isoforms in skeletal muscle explains the phenotypic variability in the rostrocaudal muscular dystrophy mouse
Wu G, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1801(4), 446-454 (2010)
Neuroprotective efficacy of mitochondrial antioxidant MitoQ in suppressing peroxynitrite-mediated mitochondrial dysfunction inflicted by lead toxicity in the rat brain
Maiti AK, et al.
Neurotoxicity Research, 31(3), 358-372 (2017)
Subcellular fractionation of mitochondria.
Rice, J.E., and Lindsay, J.G. et al.
Subcellular Fractionation, A Practical Approach, 107-142 (1997)
Arpan Kumar Maiti et al.
Interdisciplinary toxicology, 11(4), 306-315 (2019-11-26)
Nickel is a potential neurotoxic pollutant inflicting damage in living organisms, including fish, mainly through oxidative stress. Previous studies have demonstrated the impact of nickel toxicity on mitochondrial function, but there remain lacunae on the damage inflicted at mitochondrial respiratory
Arpan K Maiti et al.
Scientific reports, 5, 15434-15434 (2015-10-21)
Citrobacter rodentium is a murine pathogen that serves as a model for enteropathogenic Escherichia coli. C. rodentium infection reduced the quantity and activity of mitochondrial respiratory complexes I and IV, as well as phosphorylation capacity, mitochondrial transmembrane potential and ATP
Exibir todos os documentos relacionados

Artigos

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Mitochondrial Stress and ROS

Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica