CG200
cGMP Enzyme Immunoassay Kit, Direct
sufficient for 96 assays
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Produtos recomendados
uso
sufficient for 96 assays
Nível de qualidade
técnica(s)
ELISA: suitable
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Informações sobre genes
human ... PRKG1(5592) , PRKG2(5593)
Aplicação
Non-radioactive, competitive immunoassay for the quantitation of total cGMP in tissue and cell cultures. Buffer contains 0.1N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.
Please refer to the attached Protocolfor details.
Somente componentes do kit
Nº do produto
Descrição
- 0.1 M Hydrochloride Acid 1 x 30
- Acetic Anhydride 1 x 1
- cGMP-Alkaline Phosphatase Conjugate 1 x 5
- cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5
- Cyclic GMP Standard 1 x 0.5
- Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea
- Neutralizing Reagent 1 x 6
- p-Nitrophenyl Phosphate Substrate Solution 1 x 20
- Plate Sealer 1 ea
- Stop Solution 1 x 5
- Triethylamine 1 x 2
- Wash Buffer Concentrate 1 x 30
Ver tudo (12)
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
Órgãos-alvo
Respiratory system
Perigos de suplementos
Código de classe de armazenamento
3 - Flammable liquids
Ponto de fulgor (°F)
120.2 °F
Ponto de fulgor (°C)
49 °C
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Madiha A H Hassan et al.
BMC pharmacology, 5, 10-10 (2005-04-09)
Cardiac hypertrophy is a major risk factor for morbidity and mortality in a number of cardiovascular diseases. Consequently, the signaling pathways that inhibit cardiac hypertrophy are currently receiving much interest. Among them, nitric oxide (NO), signaling via cGMP and cGMP-dependent
Chard, T.
An Introduction to Radioimmunoassay and Related Techniques (1990)
Tijssen, P.
Practice and Theory of Enzyme Immunoassays (1985)
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