Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos

CG200

Sigma-Aldrich

cGMP Enzyme Immunoassay Kit, Direct

sufficient for 96 assays

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 96 assays

Nível de qualidade

200

técnica(s)

ELISA: suitable

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... PRKG1(5592) , PRKG2(5593)

Aplicação

Non-radioactive, competitive immunoassay for the quantitation of total cGMP in tissue and cell cultures. Buffer contains 0.1N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.
Please refer to the attached Protocolfor details.

Somente componentes do kit

Nº do produto
Descrição
  • 0.1 M Hydrochloride Acid 1 x 30
  • Acetic Anhydride 1 x 1
  • cGMP-Alkaline Phosphatase Conjugate 1 x 5
  • cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5
  • Cyclic GMP Standard 1 x 0.5
  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea
  • Neutralizing Reagent 1 x 6
  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20
  • Plate Sealer 1 ea
  • Stop Solution 1 x 5
  • Triethylamine 1 x 2
  • Wash Buffer Concentrate 1 x 30
Ver tudo (12)

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3

Órgãos-alvo

Respiratory system

Perigos de suplementos

EUH071

Código de classe de armazenamento

3 - Flammable liquids

Ponto de fulgor (°F)

120.2 °F

Ponto de fulgor (°C)

49 °C

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Lance M Wheeler et al.
Nature communications, 8(1), 1722-1722 (2017-11-25)
Materials with switchable absorption properties have been widely used for smart window applications to reduce energy consumption and enhance occupant comfort in buildings. In this work, we combine the benefits of smart windows with energy conversion by producing a photovoltaic
Yuichiro Kunai et al.
Journal of the American Chemical Society, 139(43), 15328-15336 (2017-10-08)
The concept of electrical energy generation based on asymmetric chemical doping of single-walled carbon nanotube (SWNT) papers is presented. We explore 27 small, organic, electron-acceptor molecules that are shown to tune the output open-circuit voltage (V
Madiha A H Hassan et al.
BMC pharmacology, 5, 10-10 (2005-04-09)
Cardiac hypertrophy is a major risk factor for morbidity and mortality in a number of cardiovascular diseases. Consequently, the signaling pathways that inhibit cardiac hypertrophy are currently receiving much interest. Among them, nitric oxide (NO), signaling via cGMP and cGMP-dependent
Chard, T.
An Introduction to Radioimmunoassay and Related Techniques (1990)
Tijssen, P.
Practice and Theory of Enzyme Immunoassays (1985)

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica