OE19 Cell Line human

96071721, human esophagus, Epithelial

• Sinônimo(s): JROECL19, JROECL19 Cells, OE-19 Cells, OEC19 Cells
• Código UNSPSC: 41106514

Propriedades

• product name: OE19 Cell Line human, 96071721
• fonte biológica: human esophagus
• modo de crescimento: Adherent
• cariótipo: Aneuploid
• morfologia: Epithelial
• produtos: Not specified
• receptores: MHC class I
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian oesophageal carcinoma

Descrição de linhagem celular

The cell line OE19, also known as JROECL19, was established in 1993 from an adenocarcinoma of gastric cardia/oesophageal gastric junction of a 72 year old male patient. The tumour was identified as pathological stage III (UICC) and showed moderate differentiation. The cell line OE19 expresses HLA-A, -B and -C antigens (MHC class I) constitutively. Treatment with interferon-gamma induced the expression of ICAM-1 (CD54). Expression of HLA-DR (MHC class II) on interferon-gamma addition was only measured in a sub-population of OE19. The cells express epithelial cytokeratins and are tumourigenic in nude mice.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Aplicação

OE19 cell line has been used to study the effect of microRNA-MiR-193b on autophagy and cell death on these cells. It has also been used to study microRNA-330-5p as a modulator of neoadjuvant chemoradiotherapy sensitivity.

Study of oesophageal cancer

Perfil do DNA

STR-PCR Data:
Amelogenin: X
CSF1PO: 11,13
D13S317: 9,11
D16S539: 12,13
D5S818: 11,14
D7S820: 8
THO1: 8,9
TPOX: 8
vWA: 16,17

Meio de cultura

RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:8, i.e., seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. These cells grow slowly in islands and when resuscitated from frozen it can take up to 7 days to reach 70% confluence.

Outras notas

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