NCI-H69VCR/R

96042330, human lung, Not specified

• Código UNSPSC: 41106514
• eCl@ss: 32190102

Propriedades

• product name: NCI-H69VCR/R, 96042330
• fonte biológica: human lung
• modo de crescimento: Aggregates in suspension
• cariótipo: Not specified
• morfologia: Not specified
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human small cell lung cancer, drug-resistant

Descrição de linhagem celular

NCI-H69 VCR/R has been derived from the parent small cell lung cancer line NCI-H69 (Sigma Catalogue number. 91091802) It is recommended to culture cells without drug upon resuscitation; add vincristine once growth is fully established. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Aplicação

Drug-resistance studies

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17

Meio de cultura

RPMI 1640 + 2mM Glutamine + 2.0 μg/ml vincristine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 3-9x 100,000 cells/ml; 5% CO₂; 37ºC. Resuscitate in drug-free medium, add after growth has been established.

Outras notas

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