B16 melanoma 4A5 Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94042254, mouse skin, Fibroblast-like, Mouse melanoma, producing melanin

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: mouse skin
• embalagem: tube of 5 μg 94042254-DNA-5UG; pkg of vial of cells 94042254-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fibroblast-like
• produtos: Melanin
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse melanoma, melanin producing

Descrição de linhagem celular

Derived from a melanoma from the skin of a C57BL/6 strain mouse, showing fibroblast-like characteristics, which produces melanin. Cells may lose ability to produce pigmentation in long term culture.

Aplicação

B16 melanoma 4A5 cell line has been used to study the spectral and biological characteristics of copper (II) mesoporphyrinic complexes. It has also been used to evaluate the cytotoxicity of JaZ-30, a matrix metalloproteinase-2 (MMP-2) inhibitor.

Meio de cultura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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