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Key Documents

106-05N

Sigma-Aldrich

Human Dermal Fibroblasts: HDF, neonatal

Sinônimo(s):

HDF cells

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About This Item

Código UNSPSC:
41106514
NACRES:
NA.81

fonte biológica

human neonatal foreskin or adult skin (normal)

Nível de qualidade

embalagem

pkg of 500,000 cells

fabricante/nome comercial

Cell Applications, Inc

modo de crescimento

Adherent

cariótipo

2n = 46

morfologia

Fibroblast

técnica(s)

cell culture | mammalian: suitable

Condições de expedição

dry ice

temperatura de armazenamento

−196°C

Descrição geral

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)

Origem de linhagem celular

Skin

Aplicação

injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response

Componentes

Fibroblast Basal Medium containing 10% FBS & 10% DMSO

Nota de preparo

  • Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 16 doublings

Rotina de subcultura

Please refer to the HDF Culture Protocol.

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Protocolos

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

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