GS-109-V-20

90110505, human skin, Fibroblast-like

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: GS-109-V-20, 90110505
• fonte biológica: human skin
• embalagem: tube of 5 μg 90110505-DNA-5UG; pkg of vial of cells 90110505-1VL
• modo de crescimento: Adherent
• cariótipo: 2n = 46
• morfologia: Fibroblast-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian skin fibroblast Gardner′s syndrome

Descrição de linhagem celular

Derived from skin from the upper arm of an 18 year old Caucasian male with Gardner′s syndrome is an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. Cells exist in an initiated state rather than a normal or transformed state. Normal diploid karyotype.

Aplicação

Tumourigenicity studies

Meio de cultura

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Monolayer will form in a 25cm² flask in 1 week.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.