NCTC 2071

90050803, mouse connective tissue, Fibroblast

• Código UNSPSC: 41106514
• fonte biológica: mouse connective tissue
• modo de crescimento: Adherent
• cariótipo: Modal no. 63
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified

Propriedades

• Nome do produto: NCTC 2071, 90050803
• fonte biológica: mouse connective tissue
• modo de crescimento: Adherent
• cariótipo: Modal no. 63
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse C3H/An connective tissue

Descrição de linhagem celular

Derivative of NCTC clone 929 and was established in chemically defined medium (NCTC 109). It was subsequently adapted to cysteine-free medium (NCTC 135). The cells have been used in nutritional, immunological and toxicological studies; nutritional, metabolic and enzyme studies; preservation and storage of viable tissue; fluid suspension culture methods and replicate culture techniques and quantitative cell culture methods.

Aplicação

Nutritional, immunological and toxicological studies

Meio de cultura

NCTC 135 100% + 2mM Glutamine.

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 5x10,000 to 2x100,000 cells/cm² by scraping off the cells and resuspending into new flasks; 5% CO₂; 37°C. Freeze in 9% glycerol/91% FBS.

Outras notas

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