AV3 (HeLa derivative)

88102402, human cervix, Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: AV3 (HeLa derivative), 88102402
• fonte biológica: human cervix
• modo de crescimento: Adherent
• cariótipo: 2n = 46
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human cervix carcinoma

Descrição de linhagem celular

This cell line was originally derived from normal human amnion. The cells are mainly used for virus studies (susceptible to poliovirus 1). HeLa marker chromosomes and type A G6PD have been detected. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line must be considered to be derived from HeLa.

Aplicação

Virus studies: poliovirus 1

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 12
THO1: 7
TPOX: 8,12
vWA: 16,18

Meio de cultura

EMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x100,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Outras notas

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