A9HT

88042804, mouse unknown/unspecified, Fibroblast

• Código UNSPSC: 41106514

Propriedades

• product name: A9HT, 88042804
• fonte biológica: mouse unknown/unspecified
• modo de crescimento: Adherent
• cariótipo: Hyperdiploid
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

A9 tumour induced C3H mouse

Descrição de linhagem celular

This cell line was selected by passaging A9 (Sigma Catalogue number. 85011426) through syngenic C3H or semi allogenic mice over a period of three months and uptake was considerably higher than A9, thus A9 high uptake or A9HT. Karyotype is similar to A9 but with a slightly reduced total number of chromosomes, with the modal number of 53. This line lacks inosinic acid pyrophosphorylase.

Aplicação

Tumourigenicity studies

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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