648310-M

Tris(hidroximetil)-aminometano

≥99% (perchloric acid titration), solid, pH 7.0-9.0, solid, Molecular Biology Grade

• Sinônimo(s): 2-amino-2-(hidroximetil)-1,3-propanodiol, THAM, Tris base, Trometamol
• Fórmula linear: NH₂C(CH₂OH)₃
• Número CAS: 77-86-1
• Peso molecular: 121.14
• Beilstein: 741883
• Número MDL: MFCD00004679
• Código UNSPSC: 12161705
• NACRES: NA.25

Propriedades

• Nome do produto: Tris(hidroximetil)-aminometano, TRIS base is useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
• grau: Molecular Biology
• Nível de qualidade: 100
• descrição: Merck USA index - 14, 9772
• Ensaio: ≥99% (perchloric acid titration)
• Formulário: solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• cor: white
• faixa de pH útil: 7-9
• pKa (25 °C): 8.1
• p.e.: 219-220 °C/10 mmHg (lit.)
• pf: 167-172 °C (lit.)
• solubilidade: water: 500 mg/mL
• traços de cátion: heavy metals: ≤0.0005%
• Absorção: ≤0.02 at 280 nm; ≤0.03 at 260 nm
• atividade externa: RNases, DNases, proteases, none detected
• temperatura de armazenamento: 10-30°C
• cadeia de caracteres SMILES: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• chave InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N

Descrição

Descrição geral

Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used as a biological buffer (absorbance: ≤0.03 at 260 nm; ≤0.02 at 280 nm). Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Aplicação

• A Fast and Automated Melanin-bleaching Method for Histopathologic Evaluation of Pigmented Melanoma Tissues.: This study presents an automated melanin-bleaching technique using a Tris-based buffer to enhance histopathologic evaluation of pigmented melanoma tissues, aiding in more accurate diagnoses (Ugolini et al., 2022).


• Improvement of plant protein solubilization and 2-DE gel resolution through optimization of the concentration of Tris in the solubilization buffer.: Highlights the optimization of Tris concentration in solubilization buffers to enhance protein solubilization and gel electrophoresis resolution, crucial for biochemical studies (Cho et al., 2010).

Advertência

Toxicity: Irritant (B)

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable