HL60 Ast.4

88120802, human blood, Promyeloid, blast/promyelocyte

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: HL60 Ast.4, 88120802
• fonte biológica: human blood
• embalagem: tube of 5 μg 88120802-DNA-5UG; pkg of vial of cells 88120802-1VL
• modo de crescimento: Suspension
• cariótipo: 2n = 48, hyperdiploid
• morfologia: Promyeloid, blast/promyelocyte
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian promyelocytic leukaemia

Descrição de linhagem celular

Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Treatment with 50nM TPA results in only very limited differentiation towards basophilic morphology (Giemsa stain). Differentiation towards monocyte lineage has not been observed.

Aplicação

Differentiation studies

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

Rotina de subcultura

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Do not allow the culture medium to become acidic.

Outras notas

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