WEHI 164 Cell Line from mouse

87022501, mouse skin (fibroblast), Fibroblast

• Código UNSPSC: 41106514

Propriedades

• product name: WEHI 164 Cell Line from mouse, from mouse skin(fibroblast), 87022501
• fonte biológica: mouse skin (fibroblast)
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse BALB/c fibrosarcoma

Descrição de linhagem celular

Established from a fibrosarcoma induced by subcutaneous injection of methylcholanthrene in BALB/c mice. Highly sensitive after pre-treatment with actinomycin D to human cytotoxic monocytes, to tumour necrosis factor and to lymphotoxin. It provides convenient target cells for short-term (51) chromium release assays.

Aplicação

Tumourigenicity and cytotoxicity studies, chromium release assays

Meio de cultura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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