LS180

87021202, human colon, Epithelial-like

• Código UNSPSC: 41106514

Propriedades

• product name: LS180, 87021202
• fonte biológica: human colon
• modo de crescimento: Adherent
• cariótipo: Modal no. 45 (2n=46)
• morfologia: Epithelial-like
• produtos: Carcinoembryonic antigen (CEA), Mucin
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian colon adenocarcinoma

Descrição de linhagem celular

Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon. The cells secrete CEA and mucin and are tumorigenic in nude mice. LS174T (Sigma Catalogue number. 87060401) was derived from the same patient. The cells have not been exposed to trypsin.

Aplicação

Tumourigenicity studies

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² by scraping; 5% CO₂; 37°C.

Outras notas

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