V79-HG04

87020406, hamster lung, Fibroblast-like

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: V79-HG04, 87020406
• fonte biológica: hamster lung
• embalagem: tube of 5 μg 87020406-DNA-5UG; pkg of vial of cells 87020406-1VL
• modo de crescimento: Adherent
• cariótipo: Aneuploid
• morfologia: Fibroblast-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Hamster Chinese embyro lung fibroblast transformed

Descrição de linhagem celular

A spontaneously transformed cell line of a primary culture derived from embryonic lung fibroblasts of a Chinese hamster. Thioguanine resistant, HAT sensitive, high cloning efficiency in soft agar and used for HPRT- genotyping selection in HAT.

Aplicação

Cell fusion studies

Meio de cultura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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