GH3

87012603, rat brain, Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: GH3, 87012603
• fonte biológica: rat brain
• modo de crescimento: Adherent
• cariótipo: Hyperdiploid, modal no. 60
• morfologia: Epithelial
• produtos: Growth hormone, prolactin
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat Wistar-Furth pituitary tumour

Descrição de linhagem celular

Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.

Aplicação

Hormone studies

Meio de cultura

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flask

Outras notas

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