NRK-49F

86101301, rat kidney, Fibroblast

• Código UNSPSC: 41106514

Propriedades

• product name: NRK-49F, 86101301
• fonte biológica: rat kidney
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat kidney fibroblast

Descrição de linhagem celular

Derived from normal kidney and exhibits contact-inhibition. It is sensitive to viral or chemical transformation, including proteins such as SGF. Originated from the same mixed culture as NRK-52E but has distinct characteristics.

Aplicação

Contact inhibition and transformation studies

Meio de cultura

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5-10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. The cells should be maintained sub-confluent, otherwise they will transform.

Outras notas

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