Hs 578T

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86082104, human breast, Epithelial

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human breast
• embalagem: tube of 5 μg 86082104-DNA-5UG; pkg of vial of cells 86082104-1VL
• modo de crescimento: Adherent
• cariótipo: 2n = 46
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human breast carcinoma

Descrição de linhagem celular

Tumorigenic in immunosuppressed mice and form colonies in semisolid medium. Neither oestrogen receptors nor endogenous viruses were detected.

Aplicação

Hs 578T has been used to measure the calcium-dependent binding of cadherin-11 and N-cadherin. It has also been used to study the changes in gene expression induced by insulin-like growth factor-binding protein (IGFBP-2).

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,12
D5S818: 11
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 17

Meio de cultura

DMEM + 2mM Glutamine + 10μg/ml Bovine Insulin + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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