Hep2 (HeLa derivative) Cell Line human

86030501, human cervix, Epithelial

• Código UNSPSC: 41106514
• fonte biológica: human cervix
• modo de crescimento: Adherent
• cariótipo: Modal no. 14
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• doença(s) relevante(s): cancer

Propriedades

• Nome do produto: Hep2 (HeLa derivative) Cell Line human, 86030501
• fonte biológica: human cervix
• descrição: Human Black, cervix carcinoma
• modo de crescimento: Adherent
• cariótipo: Modal no. 14
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human cervix carcinoma

Descrição de linhagem celular

Originated from tumours produced in irradiated-cortisonised weanling rats after injecting with epidermoid carcinoma tissue from the larynx of a 56 year old male. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

Aplicação

Human Hep2 (HeLa derivative) cell line has been used for virus and tumorigenicity studies.

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. After thawing the first subculture may take 7-10 days.

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