WI 38VA13 Subline 2RA

85062512, human lung (fetal), Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: WI 38VA13 Subline 2RA, 85062512
• fonte biológica: human lung (fetal)
• modo de crescimento: Adherent
• cariótipo: 2n = 46, hyperdiploid, modal no. 73-78
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian foetal lung, SV40 transformed

Descrição de linhagem celular

An SV40 transformed derivative of WI 38. The cells are characterised by loss of contact inhibition, unlimited proliferation and the presence of SV40 antigens.

Aplicação

Transformation and virus studies: SV40

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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