8805209

BER human cell line

IHW Number: 9093

• Código UNSPSC: 41106514
• NACRES: NA.81

Propriedades

• fonte biológica: human blood
• Nível de qualidade: 100
• Formulário: liquid
• embalagem: tube of 5 μg 8805209-DNA-5UG; pkg of vial of cells 8805209-1VL
• etnia: German
• sexo: male
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblastoid

Descrição

Descrição geral

Consanguineous: No

Descrição de linhagem celular

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Embalagem

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Perfil do DNA

A: 201
Cw: 602
B: 1302
HLA DRA: 101
HLA DRB1: 701
HLA DRB4: 101
HLA DQAP: 2.1
HLA DQA1: 201
HLA DQB1: 201
HLA DPA1: 1
HLA DPB1: 02012 0401

Perfil sorológico

HLA A: 2
HLA A ief: 2.2
HLA C: 6
HLA B: 13
HLA B ief: 13.2
Bw4/bw6: 4
HLA Dw: 7
HLA DR: 7
HLA DR53: 53
HLA DQ: 2
HLA DP: 2 4

Meio de cultura

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Rotina de subcultura

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Exoneração de responsabilidade

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable