HeLa/MMTV-luc

11033106, human cervix, Epithelial

• Sinônimo(s): HeLa
• Código UNSPSC: 41106514

Propriedades

• Nome do produto: HeLa/MMTV-luc, 11033106
• fonte biológica: human cervix
• embalagem: tube of 5 μg 11033106-DNA-5UG; pkg of vial of cells 11033106-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice

Descrição

Origem de linhagem celular

HeLa, genetic modification, a firefly luciferase reporter gene, MMTV promoter

Descrição de linhagem celular

HeLa/MMTV-luc is a HeLa cell line stably transfected with a firefly luciferase reporter gene under the control of the MMTV promoter. HeLa/MMTV-luc can be used to detect glucocorticoid mediated transcriptional activity of chemicals.

Perfil do DNA

STR-PCR Data:

Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Meio de cultura

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 16 μg/ml Blasticidin S HCL.

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 2 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Outras notas

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Exoneração de responsabilidade

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.