AE17 cell line from mouse lung
10092310, mouse lung, Cells are epithelial-like
Sinônimo(s):
AE17 Cells
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About This Item
Código UNSPSC:
41106514
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Nome do produto
AE17 cell line from mouse lung, 10092310
fonte biológica
mouse lung
descrição
Mouse malignant mesothelioma
modo de crescimento
Adherent
cariótipo
Not specified
morfologia
Cells are epithelial-like
produtos
Not specified
receptores
Not specified
técnica(s)
cell culture | mammalian: suitable
doença(s) relevante(s)
cancer
Condições de expedição
dry ice
temperatura de armazenamento
−196°C
Origem de linhagem celular
Mouse malignant mesothelioma
Descrição de linhagem celular
Mice (C57Black/6J strain female 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development (both ascites and solid tumours). Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice. Cell Bank Australia reference number: CBA0156
Aplicação
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.
Meio de cultura
RPMI1640 (with 2mM L-Glutamine+25mM HEPES) + 5% FCS
Rotina de subcultura
Optimal split ratio 1:16 - 1:32 (seeding density 0.6 x104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.
Outras notas
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Richard Lake - University of Western Australia, Australia
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