AB12 cell line from mouse lung

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 10092306, mouse lung, Cells are stellate-shaped

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: mouse lung
• descrição: Malignant mesothelioma from mouse lung
• embalagem: tube of 5 μg 10092306-DNA-5UG; pkg of vial of cells 10092306-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Cells are stellate-shaped
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse malignant mesothelioma

Descrição de linhagem celular

Mice (BALB/c female 6-8 weeks) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice. Cell Bank Australia reference number: CBA0146

Aplicação

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Meio de cultura

RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 5% FCS

Rotina de subcultura

Optimal split ratio 1:16 - 1:32 (seeding density 0.8 x104 cells/cm²). Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Outras notas

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Richard Lake - University of Western Australia, Australia