CAD cell line from mouse

08100805

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: mouse nerve
• descrição: Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumor
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Neuronal
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumour

Descrição de linhagem celular

CAD cells (Cath.-a-differentiated) are a variant of a CNS catecholaminergic cell line established from a brain tumour in a transgenic mouse carrying wild-type SV40 T antigen under transcriptional control of rat tyrosine hydroxylase promoter. CAD has lost the immortalising oncogene and expresses neurone-specific proteins and synaptic vesicle proteins. CAD will differentiate to form neuronal processes in serum free conditions.

Aplicação

Study of neuronal differentiation

Meio de cultura

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 8% Foetal Bovine Serum (FBS)

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 X 10⁴ cells/cm² Cells should be dislodged by gentle pipetting; 5% CO₂; 37°C.

Outras notas

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