JU77 cell line human

10092309, human lung, epithelioid: spindly with few vacuoles. Cobblestone morphology at confluency.

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: JU77 cell line human, 10092309
• fonte biológica: human lung
• descrição: Malignant mesothelioma cells from human lung
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial-like: cells are spindle-shaped with few vacuoles. Upon confluence develop an epithelioid "cobblestone-like mat"
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human malignant mesothelioma

Descrição de linhagem celular

This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. JU77 is not tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0140

Aplicação

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Meio de cultura

RPMI 1640 (with 2mM L-Glutamine+ 25mM HEPES) + 5% FCS

Rotina de subcultura

Optimal split ratio 1:8 (seeding density 0.8 x104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Outras notas

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Depositor and originator: Richard Lake - University of Western Australia, Australia