Mero-25

9100102, human lung, Mixed epithelial and spindle shaped

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: Mero-25, 09100102
• fonte biológica: human lung
• modo de crescimento: Adherent
• cariótipo: Number of chromosomes 127-140
• morfologia: Mixed epithelial and spindle shaped
• produtos: Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Descrição de linhagem celular

Mero-25 was derived from autopsy tumour material from a male patient aged >60 with a history of asbestos exposure

Aplicação

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods

Perfil do DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 11,12
D5S818: 11,12
D7S820: 9
THO1: 8
TPOX: 9
vWA: 16,17

Meio de cultura

Hams F10 + 15% FCS + 2mM Glutamine

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 48 hours

Outras notas

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