C6-2-3

8062528, rat brain, fibroblastic

• Código UNSPSC: 41106514

Propriedades

• product name: C6-2-3, 08062528
• fonte biológica: rat brain
• modo de crescimento: Adherent
• cariótipo: modal No varied with passage see reference
• morfologia: fibroblastic
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat glioma x rat glioma hybrid

Descrição de linhagem celular

A pseudotetraploid call line generated by fusion of 8-bromodeoxyuridine-resistant mutant rat glioma cell line C6-BU-1 and the 6-thioguanine-resistant mutant rat glioma cell line C6-TG-1.

Aplicação

electrophysiology

Meio de cultura

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C

Outras notas

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