T7

7021402, mouse lung, Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: T7, 07021402
• fonte biológica: mouse lung
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse Type-II lung epithelial cell

Descrição de linhagem celular

Fully differentiated Type-II alveolar epithelial cell line derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33 - 35°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only.

Meio de cultura

DMEM + 2mM Glutamine + 4% Foetal Bovine Serum (FBS) + 8 additional supplements - please see ′Additional Literature Report′ field below for full details. ECACC can supply an information sheet on the preparation of the supplements on request - contact ECAC

Rotina de subcultura

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;10% CO₂; 33°C. Doubling time 48h

Outras notas

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