LA-N-2

6041202, human nerve, Large elongated cells + small round cells in clusters

• Código UNSPSC: 41106514

Propriedades

• product name: LA-N-2, 06041202
• fonte biológica: human nerve
• modo de crescimento: Not specified
• cariótipo: Modal no. 73, range 46 - 73
• morfologia: Large elongated cells + small round cells in clusters
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Neuroblastoma

Descrição de linhagem celular

Established by Seeger et al., (1977) from the primary site of a noncatecholamine-producing neuroblastoma of a 3-year-old female with clinical Stage IV neuroblastoma. The culture consists mostly of large, elongated cells with processes with some small round cells adhering to the elongated cells. The cells are tumourigenic in nude mice.

Meio de cultura

EMEM (with non-essential amino acids) and Ham′s F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Rotina de subcultura

For routine maintenance, split cultures after they have become dense i.e. once every 3-4 weeks at a 1:20 to 1:50 ratio; 8% CO₂; 37oC. The culture consists of large elongated cells with processes and small round cells. The latter tend to grow in dense clusters on the former and detach readily. Most cells are moderately adherent. Remove attached cells from the substrate with trypsin/EDTA. Cells will detach in 5-10 minutes. When resuscitated from a frozen ampoule cells may appear dead after a day but reattach and resume growth within 2-3 days. Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 - 7.2; they do not tolerate more alkaline pH well. Cultures hold well at high density with periodic medium changes.

Outras notas

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