COS-7-InVitrus

5063001, African green monkey kidney, Fibroblast

• Código UNSPSC: 41106514

Propriedades

• product name: COS-7-InVitrus, 05063001, from African green monkey kidney, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• fonte biológica: African green monkey kidney
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fibroblast
• receptores: Not specified

Descrição

Origem de linhagem celular

Monkey African green kidney, SV40 transformed, serum-free

Descrição de linhagem celular

COS-7 cell line (Sigma catalog no. 87021302) adapted to grow in the chemically defined protein- and peptide-free InVitrus medium (Cell Culture Technologies). This cell line is substrate dependent, i.e., adherent. COS-7 was derived from CV-1 simian cells transformed by an origin-defective mutant of SV40.

Meio de cultura

InVitrus medium (Cell Culture Technologies http://www.cellculture.com/) + 4 mM L-Glutamine (G7513) + 5 ug/ml Insulin. When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO (D2650).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2, i.e., seeding at 5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Please note: As the cell culture medium does not contain protein it will not inactivate trypsin. Once cells have detached from the flask it is necessary to dilute the trypsin with the cell culture medium, centrifuge the cells and resuspend in fresh medium. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.