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C3117

Sigma-Aldrich

Monoclonal Anti-Cy3/Cy5−Biotin antibody produced in mouse

~1 mg/mL, clone CY-96, purified immunoglobulin, buffered aqueous solution

Sinônimo(s):

Monoclonal Anti-Cy3/Cy5

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

mouse

conjugado

biotin conjugate

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

CY-96, monoclonal

forma

buffered aqueous solution

concentração

~1 mg/mL

técnica(s)

direct ELISA: suitable
dot blot: suitable
immunocytochemistry: 1-2 μg/mL using chicken fibroblasts
microarray: suitable

Isotipo

IgG2a

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

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Descrição geral

Monoclonal Anti-Cy3/Cy5 (mouse IgG2a isotype), Biotin conjugate is derived from the CY-96 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a mixture of proteins labelled with cyanine 3 (Cy3) or Cy5. Cyanine dyes are ionized polymethine dyes.

Especificidade

The antibody recognizes Cy3 and Cy5 conjugated to proteins.

Imunogênio

mixture of proteins labeled with Cy3/Cy5.

Aplicação

Monoclonal Anti-Cy3/Cy5 Biotin antibody produced in mouse has also been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • dot blot assay
  • protein microarrays
  • immunofluorescenc

Monoclonal Anti-Cy3/Cy5-Biotin antibody produced in mouse was used to label miR-107 in human breast cancer cell lines by confocal microscopy.

Ações bioquímicas/fisiológicas

Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.
Cyanine dyes are small, soluble in aqueous solution and has tolerance to dimethyl sulfoxide (DMSO). They are more photostable than fluorescein, has high molar extinction coefficients and favourable quantum yields. Monoclonal antibodies to cyanine 3 (Cy3) and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Gieseking RL, et al.
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Quantitative profile of the uropathogenic Escherichia coli outer membrane proteome during growth in human urine
Alteri CJ and Mobley HLT
Infection and Immunity, 75(6), 2679-2688 (2007)
N Duval et al.
The Journal of cell biology, 118(3), 641-653 (1992-08-01)
We analyzed the production of Torpedo marmorata acetylcholinesterase (AChE) in transfected COS cells. We report that the presence of an aspartic acid at position 397, homologous to that observed in other cholinesterases and related enzymes (Krejci, E., N. Duval, A.
D M Mottola et al.
The Journal of pharmacology and experimental therapeutics, 262(1), 383-393 (1992-07-01)
The present work provides a detailed pharmacological characterization of dihydrexidine (DHX) (trans-10,11-dihydroxy- 5,6,6a,7,8,12b-hexahydrobenzo[a]phenanthridine), the first high-potency, full efficacy, bioavailable D1 dopamine receptor agonist. DHX represents a new conformationally rigid structural class of dopamine receptor ligands. It competes stereoselectively and potently
Pai-Sheng Chen et al.
The Journal of clinical investigation, 121(9), 3442-3455 (2011-08-16)
MicroRNAs (miRNAs) influence many biological processes, including cancer. They do so by posttranscriptionally repressing target mRNAs to which they have sequence complementarity. Although it has been postulated that miRNAs can regulate other miRNAs, this has never been shown experimentally to

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