Pular para o conteúdo
Merck
Todas as fotos(1)

Key Documents

View All Documentation

BCK-FC555-100

Sigma-Aldrich

EdU Flow Cytometry Kit 555

sufficient for 100 assays

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Código UNSPSC:
12161503
NACRES:
NA.22

fluorescência

λex 546 nm; λem 579 nm

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Procurando produtos similares? Visita Guia de comparação de produtos

Descrição geral

The Baseclick EdU Flow Cytometry Kits provide a superior alternative to BrdU and [3H]thymidine assays for directly measuring DNA synthesis. EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU Flow Cytometry Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU Flow Cytometry Kits utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.

Aplicação

Flow Cytometry

Palavra indicadora

Danger

Classificações de perigo

Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Sens. 1

Código de classe de armazenamento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a

Artigos

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica