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Documentos Principais

ABK0018

Sigma-Aldrich

AfliBridge ELISA

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About This Item

Código UNSPSC:
41116158
NACRES:
NA.84

forma do anticorpo

purified antibody

Nível de qualidade

reatividade de espécies

mouse, human

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Descrição geral

A Protein Conformational Array (PCA) in a sandwich ELISA format where the plate is coated with a panel of antibodies raised against peptides derived from the full-length protein sequence of Aflibercept. Taken individually, each of these antibodies is strongly antigenic to the peptide sequence that was used in its production. However, when these peptides are incorporated into a full length correctly folded protein, the antigenicity of many of them is masked by the three-dimensional structure of the protein and only a limited number of the antibodies respond. The result is a histogram which can be likened to a ‘fingerprint′ for correctly folded Aflibercept. For a Eylea Biosimilar, if the protein is correctly folded and glycosylated, the ‘fingerprint′ will match that of Aflibercept. If it is not correctly folded, previously masked peptide sequences will be exposed and will be recognized by the antibody made to that exposed sequence. In this way, changes in the ‘fingerprint′ generated by the ELISA will point out differences between the Biosimilar and authentic Aflibercept.

Aplicação

For research use only. Not for use in diagnostic procedures.

Outras notas

ELISA kit contains 3 96-well microplates and all the components necessary to complete an ELISA test.

Pictogramas

Corrosion

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Código de classe de armazenamento

8B - Non-combustible corrosive hazardous materials

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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